Occurrence and diversity of Listeria spp. in seafood processing plant environments

Listeria contamination in processing plant environments is a major issue for the seafood industry worldwide; faster and more reliable results are therefore desired for early detection and monitoring of environmental Listeria spp. This study aimed to gain a better understanding of the prevalence and diversity of Listeria spp., and to evaluate a rapid detection method, the 3M Molecular Detection Assay (MDA) Listeria, for its ability to detect Listeria spp. in environmental samples from seafood processing plants. Duplicate environmental sponge samples (n = 444) were collected from 152 different sites within three seafood processing plants, and analyzed for Listeria spp. by the MDA method (after 26 and 48 h of enrichment) and the U.S. Food and Drug Administration Bacteriological Analytical Manual method. Overall, detection of Listeria spp. by the two methods did not differ significantly (p > 0.05); 11 (4.9%) and 13 (5.9%) samples were positive for Listeria spp. by the MDA and FDA-BAM method, respectively. The sensitivity of the MDS was 87.0% (95% CI: 77.4–96.6%), specificity was 97.6% (95% CI: 95.5–99.7%), accuracy was 95.3%, and the positive predictive value was 89.4% (95% CI: 80.5–98.2%). Classification of 19 Listeria isolates by partial SigB sequencing analysis identified three allelic types. Twelve of these isolates were ATs 58 and 60 which were classified as Listeria monocytogenes lineage I and serotypes 1/2b, 3b, 4b, 4d, 4e, by multiplex-PCR serotyping. Six Listeria isolates were classified as Listeria innocua (AT31). Our data show that the 3M Molecular Detection Assay Listeria provides rapid and reliable results for detection and monitoring of Listeria spp., which are important for seafood processing plants. Effective Listeria monitoring programs will allow for improved development of Listeria control measures in order to minimize cross-contamination in finished products.     

Using retrospective sampling to estimate models of relationship status in large longitudinal social networks

Estimation of longitudinal models of relationship status between all pairs of individuals (dyads) in social networks is challenging due to the complex inter-dependencies among observations and lengthy computation times. To reduce the computational burden of model estimation, a method is developed that subsamples the “always-null” dyads in which no relationships develop throughout the period of observation. The informative sampling process is accounted for by weighting the likelihood contributions of the observations by the inverses of the sampling probabilities. This weighted-likelihood estimation method is implemented using Bayesian computation and evaluated in terms of its bias, efficiency, and speed of computation under various settings. Comparisons are also made to a full information likelihood-based procedure that is only feasible to compute when limited follow-up observations are available. Calculations are performed on two real social networks of very different sizes. The easily computed weighted-likelihood procedure closely approximates the corresponding estimates for the full network, even when using low sub-sampling fractions. The fast computation times make the weighted-likelihood approach practical and able to be applied to networks of any size.     

Fault estimation and accommodation for networked control systems with nonuniform sampling periods

This paper deals with the problem of fault estimation and accommodation for a class of networked control systems with nonuniform uncertain sampling periods. Firstly, the reason why the adaptive fault diagnosis observer cannot be applied to networked control systems is analyzed. Based on this analysis, a novel robust fault estimation observer is constructed to estimate both continuous‐time fault and system states by using nonuniformly discrete‐time sampled outputs. Furthermore, using the obtained states and fault information, a nonuniformly sampled‐data fault tolerant control law is designed to preserve the stability of the closed‐loop system. The proposed scheme can not only guarantee the impact of continuous‐time uncertainties and discrete‐time sampled estimation errors on the faulty system to satisfy a H _∞ performance index but also repress the negative effect of the unknown intersample behavior of continuous‐time fault by use of an inequality technique. Finally, simulation results are included to demonstrate the feasibility of the proposed method. Copyright © 2014 John Wiley & Sons, Ltd.     

The Topology Sampling of H2SO4·NH3 with Meta-Dynamics Method

The configurations of molecular clusters have significant impacts on their growth into fine particles in atmosphere. In this paper, we explore the topology space of the structure of H2SO4·NH3 dimer with a novel sampling technique of meta-dynamics (MTD) method and ab initio molecular dynamics simulations. The simulations are carried out at the temperatures of both 50 K and 242 K, which represent the typical high and low latitudes of troposphere. The results show that, compared with only traditional MD simulations, the structure samplings are significantly accelerated with MTD method. Therefore, more isomers of the dimer are discovered within the same simulation time scale. In addition, the results show that MTD is more efficient for circumstances with high temperature.     

基于Marr小波改进的SIFT算法的遥感影像配准

〖HTH〗通讯作者〖HTSS〗:[ZK（]金〓燕(1991-)，女，山东济宁人，硕士研究生，主要从事图形图像处理，图像与视觉信息计算方面的研究。E\|mail:jyan0529@163.com。[ZK）] 〖ZW)〗〖HT〗 〖AM〗〖HT5SS〗〖MM(〗〖ZZ(S〗〖HT5”〗〖SX(B〗第34卷〓第3期〖〗2019年6月〖SX)〗[KG0.2mm]〖KG7*3〗〖HT〗〖SX(B〗遥〓感〓技〓术〓与〓应〓用〖〗〖WT5，6〗REMOTE SENSING TECHNOLOGY AND APPLICATION〖SX)〗〓〓〓〓〖KG6*2〗〖WT5”BX〗〖SX(B〗Vol.34〓No.3〖〗Jun.2019〖WT〗〖SX)〗〖ZZ)〗〖MM)〗〖HT〗 〖HT2H〗〖JZ(〗〖WTHZ〗〖STHZ〗 基于Marr小波改进的SIFT算法的遥感影像配准 〖STBZ〗〖WTBZ〗〖HT4K〗 张海涛，金〓燕，刘万军 〖HT5K〗 (辽宁工程技术大学 软件学院，辽宁 葫芦岛〓125105) 〖JZ)〗〖ＨＴ５Ｈ〗〖GK2!2〗摘要〖HTK〗: [KG(0.1mm]针对遥感图像配准方法中错误匹配点对过多、配准效率低和其他性能，提出了一种基于小波的遥感图像配准方法。首先，利用尺度空间理论下的Marr小波对参考图像和待配准图像进行特征提取，然后利用欧氏距离对参考图像和待配准图像的特征点进行初配准，再根据随机采样一致法，对初配准结果进行精配准。为了验证方法的有效性，选择无人机实时航拍图像、不同时相变化遥感图像以及遥感不同高度的遥感图像。实验结果表明：该方法与SIFT(Scale Invariant Feature Transform)算法以及其他改进SIFT算法相比可以有效剔除错误匹配点对，提高了配准精度，同时提高配准效率两倍以上。该方法可以应用于不同遥感数据源，能够有效地提高配准精度，降低配准时间。[KG)] 〖ＨＴＨ〗关〓键〓词〖ＨＴ５Ｋ〗: 遥感图像配准;Marr小波;欧氏距离;随机采样一致法 〖ＨＴＨ〗中图分类号〖HTSS〗:TP79〓〓〖HTH〗文献标志码〖HTSS〗:A〓〓〖HTH〗文章编号〖HTSS〗:1004 0323(2019)03 0622 08 〖HK〗〖HT5SS〗     

基于BP神经网络的条带刚凸特征回弹预测

为了研究核燃料组件格架的条带刚凸特征的回弹量与压边力、冲压速度、凸凹模间隙、摩擦系数等冲压工艺参数之间的关系,首先,获取包含50个GA拉丁超立方抽样的数据点以及10个随机抽样的数据点的数据集,前者作为训练集、后者作为测试集。将前者输入到BP神经网络进行训练,后者验证训练模型的精度。最后,通过响应面图研究各因素之间的交互作用以及各因素的敏感程度。结果表明:BP神经网络能够有效预测刚凸回弹量与冲压工艺参数之间的关系,相对于其他因素,压边力对回弹量的影响特别明显,冲压速度对回弹量的影响不明显,但与凸凹模间隙和摩擦系数有明显的交互作用。     

基于确定性抽样的过冷沸腾边界条件不确定性分析

本文使用Fluent软件构建数学物理模型，对DEBORA过冷沸腾基准实验进行了数值模拟，并采用确定性抽样方法对模拟沸腾流动的边界条件不确定性进行分析，计算得到了主要径向参数分布的期望值和置信区间，分析了边界条件不确定性的影响趋势。此外，还计算得到了不确定性源对部分径向参数的影响权重。结果表明，流体入口温度和壁面热流密度的不确定性对径向空泡份额的影响较大，而运行压力和流体入口温度的不确定性是影响径向液体温度计算的主要因素。     

用于厚屏蔽小探测器的蒙特卡罗模拟减方差方法研究

反应堆屏蔽计算中经常出现厚屏蔽、小探测器问题，常规蒙特卡罗方法难以有效解决。基于自动重要抽样（AIS）方法，本文提出了小探测器自动重要抽样(SDAIS)方法，并针对小探测器问题，优化了AIS方法的虚粒子赌分裂算法。该方法在自主开发的蒙特卡罗屏蔽程序MCShield上进行了实现。使用NUREG/CR-6115 PWR基准题验证该方法的正确性和计算效率。结果表明，SDAIS方法可有效地解决厚屏蔽小探测器问题，相比AIS方法及传统的重要性方法，计算效率提升1~2个量级。     

Household endotoxin reduction in the Louisa Environmental Intervention Project for rural childhood asthma

Endotoxin exacerbates asthma. We designed the Louisa Environmental Intervention Project (LEIP) and assessed its effectiveness in reducing household endotoxin and improving asthma symptoms in rural Iowa children. Asthmatic school children (N = 104 from 89 homes) of Louisa and Keokuk counties in Iowa (aged 5-14 years) were recruited and block-randomized to receive extensive (education + professional cleaning) or educational interventions. Environmental sampling collection and respiratory survey administration were done at baseline and during three follow-up visits. Mixed-model analyses were used to assess the effect of the intervention on endotoxin levels and asthma symptoms in the main analysis and of endotoxin reduction on asthma symptoms in exploratory analysis. In the extensive intervention group, dust endotoxin load was significantly reduced in post-intervention visits. The extensive compared with the educational intervention was associated with significantly decreased dust endotoxin load in farm homes and less frequent nighttime asthma symptoms. In exploratory analysis, dust endotoxin load reduction from baseline was associated with lower total asthma symptoms score (Odds ratio: 0.52, 95% confidence interval: 0.29-0.92). In conclusion, the LEIP intervention reduced household dust endotoxin and improved asthma symptoms. However, endotoxin reductions were not sustained post-intervention by residents.     

GOS+MU:一种查询对象选择新方法

在分析单一MU(Most Uncertainty)采样缺陷的基础上,提出一种"全局最优搜寻"方法 GOS(Global Optimal Search),并结合MU共同完成查询选择。GOS+MU方法中,GOS着眼全局寻找目标,在应用环境能提供的训练样本数量有限、分类器受训不充分时,该方法选择的对象学习价值高,能快速推进分类器学习进程;MU则能够在GOS采样失效情形下,利用分类器当前训练成果,选择查询不确定性最强的样本补充训练集。通过对网络商品的用户评论进行分类仿真,并比较其他采样学习方法的效果,证明了GOS+MU方法在压缩学习成本、提高训练效率方面的有效性。